Dr. Packia Nancy R

About

Clinical areas of expertise

• Bacteriology- Media preparation
• Staining
• Culture
• Anti-microbial and automations
• Automations- Bactec
• BacT Alert
• Vitek 2
• MALDI-TOF
• Bio-fire
• Virology- Extraction of RNA /DNA viruses
• RT –PCR.
• Mycobacterium-AFB staining
• Culture by LJ medium
• MGIT
• Gene Xpert
• RT -PCR
• Immunology/Serology-ELISA
• Chromatographic test
• Immuno-Fluorescence test
• spot test
• CLIA
• Immunofluorescence
• Mycology-
• KOH
• wet mounts
• Culture of yeasts and moulds-Black fungi
• Aspergillus
• Dermatophytes
• Identification of yeast and mold
• Assimilation test
• sugar test
• ascospore formation.
• Serology-Galactomannan assay
• ELISA
• AFST-disc diffusion and broth microdilution(both yeast and moulds)
• Extraction of DNA-- both yeast and mold
• PCR-polymerase chain reaction with ITS and specific primers
• Research work in molecular microbiology-
•  AFST by Broth Micro-Dilution method for both yeast and moulds
•  PCR-polymerase chain reaction with ITS and specific primer
•  RT-PCR
•  Gel electrophoresis
•  Primer designing
•  Sequencing-Sanger’s sequencing
•  Biotechnology tools-Mega x software
• Mega 11 software
• bio-edit tools
•  NCBI Blasting
•  Phylogenetic analysis
•  Maximum likelihood ratio
• maximum parsimony analysis
•  Drawing phylogenetic tree
•  Multi-locus sequence analysis (MLST)
• TRAINING UNDERGONE
• • BASIC EPIDEMIOLOGY
• STATISTICS
• SPSS AND CRITICAL APPRAISAL WORKSHOP
• • Completed online programme on Research Ethics by CITI, University of Miami, USA.
• • COMPUTER APPLICATIONS IN RESEARCH
• • RESEARCH METHODOLGY
• • MOLECULAR MYCOLOGY WORKSHOP
• • INTERNATIONAL MALASSEZIA WORKSHOP at Utrecht
• • Online Hands-on Workshop on Sangers DNA sequence and Mutation analysis
• Infectious control practice-CAHO certified Basic and advanced courses in Infectious control
• Anti-Microbial stewardship
• ISO audits
• Microbiology

Professional experience